Internal ribosome entry site within hepatitis C virus RNA
نویسندگان
چکیده
منابع مشابه
Internal ribosome entry site within hepatitis C virus RNA.
The mechanism of initiation of translation on hepatitis C virus (HCV) RNA was investigated in vitro. HCV RNA was transcribed from the cDNA that corresponded to nucleotide positions 9 to 1772 of the genome by using phage T7 RNA polymerase. Both capped and uncapped RNAs thus transcribed were active as mRNAs in a cell-free protein synthesis system with lysates prepared from HeLa S3 cells or rabbit...
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HCV infection affects ~170 million people worldwide and is a major cause of chronic hepatitis as well as hepatocellular carcinoma1. Current therapies suffer from low efficiency and serious side effects. Therefore, there is an urgent need for new antiviral agents for the treatment of HCV infection. Among the potential targets for HCV inhibitors is the highly conserved 5′ untranslated region (UTR...
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The highly conserved internal ribosome entry site (IRES) of hepatitis C virus (HCV) regulates translation of the viral RNA genome and is essential for the expression of HCV proteins in infected host cells. The structured subdomain IIa of the IRES element is the target site of recently discovered benzimidazole inhibitors that selectively block viral translation through capture of an extended con...
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Natural products that target the RNA components of bacterial ribosomes and thereby act as antibiotics that shut down microbial protein synthesis, have provided a rich source of inspiration for the design and synthesis of small-molecule ligands directed at RNA targets. 2] A prominent example of a privileged scaffold for RNA recognition occurring in natural aminoglycoside antibiotics is 2-deoxyst...
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Internal ribosomal entry sites (IRESs) are structured cis-acting RNAs that drive an alternative, cap-independent translation initiation pathway. They are used by many viruses to hijack the translational machinery of the host cell. IRESs facilitate translation initiation by recruiting and actively manipulating the eukaryotic ribosome using only a subset of canonical initiation factor and IRES tr...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1992
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.66.3.1476-1483.1992